The nested polymerase chain reaction (PCR) method was used for the amplification of the influenza A H1N1 virus hemagglutinin monoclonal antibody light-chain and heavy-chain genes. Sequence analysis of the obtained genes was then used to identify common cloning methods of the mouse immunoglobulin-kappa (Igκ) light-chain and heavy-chain variable gene regions. Twenty-two pairs of amplification pri...

The variable regions of five human immunoglobulin heavy chains of the V(H)III subgroup have been totally sequenced. Three of the heavy chains belonged to the IgG class and two to the IgA class. Examination of these sequences, and comparison with additional published heavy chain sequences, showed that a total of four hypervariable regions is characteristic of human heavy chain variable regions. ...

immunoproliferative small intestinal disease (ipsid) is the syndrome associated with mediterranean lymphoma (a rare form of non-hodgkin’s lymphoma). many of the patients diagnosed with secretory ipsid have variable level of abnormal immunoglobulins in serum or other bodily fluids, identified as truncated alpha heavy chain globulins. most cases are characterized by a loss of ability to synthesiz...

2 Superantigen-like interaction of IVIG with antibody Fab fragments cloned by phage display technology Dissertation for the award of doctor rerum naturalium bp base pairs CD cluster of differentiation CDR complementarity-determining region cfu colony forming units C H 1 first constant region of antibody heavy chain C L light chain consant region cp coat protein D diversity gene DTT dithiothreit...

Immunoproliferative small intestinal disease (IPSID) is the syndrome associated with Mediterranean lymphoma (a rare form of non-Hodgkin’s lymphoma). Many of the patients diagnosed with secretory IPSID have variable level of abnormal immunoglobulins in serum or other bodily fluids, identified as truncated alpha heavy chain globulins. Most cases are characterized by a loss of ability to synthesiz...

Heavy chains of IgG, IgA, and IgM classes of human immunoglobulins were compared by N-terminal residue determination and partial amino acid sequence analysis. A third subclass of the variable region of heavy chains was observed; an unblocked glutamic acid as the N-terminal residue is characteristic of this subclass. Our results indicated that the heavy chain variable region subclasses are not c...